Summary Mechanisms of DNA Organization Unraveled with Novel Single-Molecule Methods
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DNA mechanics as a tool to probe helicase and translocase activity
Helicases and translocases are proteins that use the energy derived from ATP hydrolysis to move along or pump nucleic acid substrates. Single molecule manipulation has proved to be a powerful tool to investigate the mechanochemistry of these motors. Here we first describe the basic mechanical properties of DNA unraveled by single molecule manipulation techniques. Then we demonstrate how the kno...
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